Home

Cell Biol Chapter 3 outline

The Flow of Genetic Information

A) history of discovery of genetic material

1) Miescher

2) Griffith

3) Avery, MacLeod, McCarty

4) Hershey and Chase (blender experiments)

B) DNA structure

C) DNA replication

D) transcription

1) RNA polymerase

a) eukaryotes = 3 types I, II, III

2) promoters - Pribnow box, TATA box

3) hnRNA

E) genetic code

1) elucidation of code

2) aspects of code

3) codon - anticodon base pairing

4) wobble

5) activation or charging of tRNA

a) aminoacyl tRNA synthetase

6) ribosomes

a) prokaryote = 30S + 50S = 70S

b) eukaryote = 40S + 60S = 80S

F) protein synthesis (translation)

1) initiation

2) elongation

3) termination

G) inhibitors of protein synthesis

H) mutations

• spontaneous vs. induced
• causes of induced
• missense, nonsense, frameshift, point
• Ames test

I) Biotechnology

• recombinant DNA
• restriction enzymes
• vectors
• Southern blotting
• PCR
• DNA fingerprinting

Home