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Lab Safety

General Lab Safety

1) Never eat, drink, apply cosmetics, or smoke in the laboratory.

2) Wear appropriate clothing (gloves, safety goggles, lab coats, etc.).

3) No bare feet or open shoes such as sandals.

4) Use pipetting devices; no mouth pipetting.

5) Make sure gas jets are off and your area is clean before leaving.

6) Dispose of syringes, needles, glass, and other sharp objects properly.

7) Use fume hoods for dangerous and irritating chemicals.

8) Never remove anything from the laboratory (chemicals, glassware, etc.), it's illegal

9) Do not return unused chemicals to reagent bottles.

10) When diluting acids -- always slowly add the acid to the water while stirring.

11) Dispose of body fluids and potential pathogens properly and decontaminate work area.

12) When working with animals -- avoid causing them unnecessary pain or discomfort.

13) Immediately report ALL accidents to the instructor.

14) Use common sense; you have a responsibility to protect yourself.

15) Don't sniff or otherwise sample chemicals.

Microbiology Lab Safety

1) Access to laboratory is limited.

2) Decontaminate work surfaces at the start and finish of working in the lab.

3) Wash hands after decontaminating work area.

4) In case of spills -- cover area with paper towels, flood with disinfectant, let sit for at least 20 minutes, notify instructor.

5) Perform all procedures carefully and slowly to minimize aerosolization.

6) Anything coming into contact with live bacteria must be autoclaved.

7) Do not wear extremely loose clothing, long hair should be tied back.

8) Avoid touching face - mouth area (keep fingers, pencils, etc. away from face).

9) Keep bench work area clean and free of materials not required for lab.

10) When using boiling water baths -- fill the beaker with deionized water and make sure the beaker contains several boiling chips.

11) When boiling tubes -- make sure cap is loose!

12) When leaving lab, ensure that gas jets and hot plates are off.

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